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The metaphase preparations from the cancer cell lines were prepared using standard techniques.
Metaphase preparations from patient peripheral lymphocytes followed a standard protocol [46].
Introduced in the 1960s, the evaluation of stained metaphase preparations from tumor cells [ 1, 2] has been widely employed in basic research as well as in clinical practice.
Metaphase preparations from Epstein Barr virus transformed lymphoblastoid cells were done by standard air‐drying technique, and fluorescent in situ hybridization (FISH) was done with labeled DNA by nick‐translation technique, essentially as described.
The BAC clone zK167C09 consistently localized near the centromere of the long arm of LG chromosome 3 in the Tu strain, but had three different hybridization patterns in the metaphase preparations from AB embryos.
Metaphase preparations from RBS cells show a loss of cohesion in the pericentric heterochromatin (PCH), leading to a separation of sister chromatids at and near the centromere (Van Den Berg and Francke, 1993).
Similar(53)
Metaphase preparation from ESCs and MEFs was as previously described (Li & Wang, 2011), but with minor modification.
Metaphase preparations were generated from >20 HS tumor biopsies from both BMD and FCR.
Metaphase preparations were obtained from established fibroblast cell lines of one male individual of Callimico goeldii and of Cebuella pygmaea, of one female individual of Callithrix argentata and of one male individual of Saimiri sciureus.
Metaphase preparations were obtained from lymphoblastoid or fibroblast cell lines of the following species: human (Homo sapiens, HSA), common chimpanzee (Pan troglodytes, PTR), gorilla (Gorilla gorilla, GGO), Borneo orangutan (Pongo pygmaeus pygmaeus, PPY).
Metaphase chromosome preparations from DGAP105 were prepared on glass slides following standard hypotonic lysis and fixation, and dehydration in a series of ethanol washes as previously described (45).
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