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In Drosophila, the only holometabolous insect for which individual MB neurons have been tracked through metamorphosis, development of the tri-lobed MB occurs in a sequential fashion, with the γ lobe forming embryonically, the α'/β' lobe developing just prior to pupation, during mid-third instar, and neurogenesis of the α/β lobe initiating at the onset of pupation [12], [16].
The identified differentially expressed genes would facilitate the understanding of metamorphosis, development and the fitness to environmental change of C. megacephala.
A comprehensive sequence resource with desirable quality was built by developmental transcriptomes of eggs, larvae and adults, enriching the genomic platform, which would facilitate the understanding of metamorphosis, development and the fitness to environmental change of C. megacephala.
They might be involved in the biosynthesis pathway of endogenous compounds, such as molting hormone (20-hydroxyecdysone, 20E is the most active form) [ 3] and juvenile hormone (JH) [ 5] that are the key factors in regulating metamorphosis, development, and reproduction.
This species has been investigated focusing on larval stages for studying the hormonal regulation of metamorphosis, development, sexual differentiation, and the impact of endocrine active compounds (EAC) [ 10– 10].
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This array, has already been employed within the Pleurogene program to investigate changes in the transcriptome profiling during gonad differentiation, growth and maturation, and during larval metamorphosis and development.
Some variation may be attributable to variation among individuals in growth (i.e., mass at metamorphosis) and development (i.e., duration of metamorphosis; [40], Table 1 and Figure 3), although the large difference in body mass at the end of the experiment mostly resulted from the longer lifespan of survivors (Figure 4).
During metamorphosis the development of novel neuronal clusters and connections could generate a new architecture susceptible to ena down-regulation, which only in time would display such defects.
We measured percentage hatching, survival from hatching to metamorphosis, and development time from hatching to metamorphosis as measures of fitness.
In the second phase, after metamorphosis, heart development re-initiates and after final differentiation, the heart starts to beat and becomes functional.
We hypothesize that some members of the OBP and CSP families may play general physiological roles as carriers or may mediate responses to ligands that are important for metamorphosis and development.
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