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The P-value of the coefficient for the linear effect of peptone concentration was 0. 0081 and trace metals solution was less than 0.0001, suggesting that these were the principal variables with significant effect on the hyperthermophilic esterase production.
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Yeast nitrogen base and PTM1 trace metals solution were purchased from the Shanghai Sangon Bioengineering Co. Ltd ,China.
At equilibrium the suspension was filtered and the metal solution was analyzed.
The pH of the trace metal solution was adjusted to 4.0 with 2 M NaOH.
After 24 h, the metal solution was filtered (pore size 0.22 μm).
The pH of the trace metal solution was adjusted to 4.0 with 2 M NaOH and heat sterilized.
The SMA on methanol in a medium with the complete metal solution was 6.5% (day 22) and 17% (day 35) higher than in a medium with the complete metal solution except nickel (Fig. 5).
Negative staining, the embedding of a specimen in a layer of dried heavy metal solution, was introduced early on as a quick and easy specimen preparation technique that significantly increases the specimen contrast.
All stock solutions of individual metals except acidified FeCl3·6H2O were heat-sterilized and the trace metals solutions were wrapped in aluminum foil and stored at 25°C until use.
The mixed base solution and the mixed metal solution were added to the reaction vessel at the rates which kept the reaction pH at 7.5 ± 0.2.
An FTIR spectrum of the coconut shell powder before and after its treatment with metal solution is shown in Figure 7.
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