For isolation of lymphocytes in the lung, lung tissues were ground with fine metal strainer, followed by digestion with HBSS containing 5% FBS, 125 U/mL collagenase I (GIBCO Inc., Grand Island, NY, USA), 60 U/mL DNase I and 60 U/mL hyaluronidase (Sigma, St Louis, MO, USA) for 60 min at 37°C.
Locate a large lidded pot with a colander or metal strainer.
Pour the contents of the pan into a metal strainer set over a metal bowl.
It's a superb natural insulator that keeps ice drier and less slushy than a metal strainer or bowl.
Pour through a fine metal strainer or cheesecloth into a small container.
Following digestion (37°C, 14 min), pancreata were broken apart by aspirating through a 14G needle, then filtered through a metal strainer (0.8 mm).
Following digestion (37°C, 14 min), pancreata were dispersed by gently aspirating through a 14G needle, then filtered through a metal strainer (0.8 mm).
Strain the syrup through a metal strainer into pitcher.
Find a fine metal strainer to remove surface burn.
