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Due to the fact that these residues are completely conserved in the protein sequences of Aminobacter sp. DSM24754 and Aminobacter sp. DSM24755 and due to the overall high sequence similarity with the well-studied dihydropyrimidinase of S. meliloti CECT4114 a metal dependence of these newly described enzymes is most likely.
(B ) Metal dependence of the B4GAT1dTM GlcA-T activity.
However, the metal dependence of hCaMKP(1 559) was somewhat different from that of CaMKP.
The relatively slow kinetics and metal dependence of IN-catalysed reallowed allowed us to freeze trap the relevant Michaelis complexes formed prior to 3′-processing and strand transfer without resorting to the use of inactivating mutations or detuned reaction conditions.
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A characterization of the B4GAT1 GlcA-T activity revealed a metal dependence for manganese (Mn2+) divalent cations and a pH-optimum near pH 7.0.
In previous work, we have demonstrated that tetrahydrobiopterin interaction and metal-dependence of alkylglycerol mono-oxygenase is similar to tetrahydrobiopterin-dependent aromatic amino acid hydroxylases, but different from nitric oxide synthases [ 11].
We describe the metal-dependence of catabody construct 2E6, an engineered heterodimer of immunoglobulin light chain variable domains that hydrolyzes amyloid β peptides (Aβ) specifically.
As one of the most significantly upregulated proteins we also sought to test the metal-dependence of this effect in vitro.
For the assessment of metal dependence, all ions were used at a concentration of 10 mM in 0.1 M MOPS pH 7.0.
Our study delineates the potential evolutionary path between Zn2+-free Zn2+-freedependent PBGS enyzmes showing thandthe rigid backbone of PBGS enzymes is an ideal framework to create or eliminate metal dependence through a limited number of amino acid exchanges.
The data on metal dependence were taken from the BRENDA database [ 185], the presence of metals was as listed in the Protein Data Bank [ 140] entries.
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