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The metal coordination is the key factor to anchor Au NPs at the PA66 surfaces after the reduction process.

It was found that controlling the metal coordination is the key parameter to template the Au NPs on the spherulite surfaces.

The field of protein crystal engineering with metal coordination is relatively new and has gained considerable attention for developing solid biomaterials as well as structural investigations of enzymatic reactions, growth of nanoparticles and catalysis.

In addition to the conformational changes observed in Asp108, another significant difference in metal coordination is observed with the bridging ligand Asp191.

In the case of linear and cyclic chelators metal coordination is maintained via free carboxylic groups and nitrogen atoms forming a 6-, 7-, 8- or 9-fold coordinated complex with one or more water molecules attached to the complex [94,95].

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First evidence for metal coordination was obtained with ligand 16 by generation of zinc and copper complexes.

For mutant MB, the Mn2+ ion adopts similar coordination geometry except that the metal-bound solvent molecule is observed only in monomer A in the asymmetric unit; in monomer B, the corresponding solvent molecule is too far from Mn2+ to be considered an inner-sphere coordination interaction, so the metal coordination geometry is classified as square pyramidal.

It has been shown in numerous reports that metal carboxylate coordination was central to their molecular packing and lattice structures.

Within the PDB_REDO pipeline, peptides with backbone oxygen or nitrogen atoms flagged by LINK-records in the PDB header (denoting they are involved in chemical interactions with other compounds, for instance in metal ion coordination) are excluded from pepflip analysis.

The nitrogen atoms of histidine imidazole groups are occasionally observed as metal ligands in Mn2+ metalloenzymes despite their borderline hardness, the metal coordination geometry is usually square pyramidal or trigonal bipyramidal (coordination number = 5) [13].

The metal coordination sphere is shown as dashed line.

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