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Water collected for major and trace metal analysis were preserved by acidifying with a few drops of HNO3 acid to achieve a pH of ≤2.
Water samples for trace metal analysis were acidified to pH <2 with ultra-purified 6 M HNO3 and stored in pre-cleaned polyethylene containers.
The water samples for heavy metal analysis were collected in 500 mL acid washed polyethylene bottles preserved by nitric acid addition at the site (pH < 2).
The samples meant for heavy metal analysis were acidified to a pH of <2 by the addition of MerckTM ultra pure nitric acid in the field.
Precision and accuracy of heavy metal analysis were assessed through repeated analysis of the samples against National Institute of Standard and Technology, Standard Reference Material (SRM 1570) for all the heavy metals.
The samples for heavy metal analysis were filtered through pre-washed 0.45 m Millipore nitrocellulose filters to remove any suspensions treated with 2 mL of Analar HNO3 at pH < 2 to preserve them.
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Metal analysis was performed as described above.
Precision of dissolved metal analysis was typically 3%.
Heavy metal analysis was performed to quantify the toxic components present in water samples.
The heavy metal analysis was accomplished using atomic absorption spectrophotometer (AAS) (Brand Varian Spectra 55B).
The procedure for heavy metal analysis is documented elsewhere (Zhong-he and Ármannsson 2005).
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