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The test centre in Balma, a suburb of Toulouse, specialises in metal analysis and is equipped with a scanning electron microscope capable of 100,000 times magnification.
The reagent blanks and known standards were used throughout the metal analysis and used to correct the analytical results.
Heavy metal analysis and local geology study support the hypothesis that the main source of chromium is of geogenic origin.
For ensuring the quality control we used the certified reference materials (CRM) for metal analysis and also performed the recovery test with the best digestion method for each metals (Table 2).
The water content in tissues was determined during preparation of samples for metal analysis and used to normalize PAH concentration (micrograms per gram) to dry weight.
In a recent review the importance of trace metal analysis and the potential of metal speciation analysis in CSF as a diagnostic tool for better understanding of neurodegenerative diseases were discussed [ 18].
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Solvents used in extraction, metals analysis and reagents for phytochemical tests were of pure analytical grade.
Aliquots of 0.6 ml of cells were transferred to treated microcentrifuge tubes prepared by the metal analysis facility and pelleted.
Physicochemical parameters and heavy metal analysis quantify different organic and inorganic pollutants and identify the toxic components, respectively.
Leaves were harvested for gene expression and metal analysis after two, eight and 14 weeks.
Samples were diluted using deionised water and filtered prior to heavy metal analysis (Zn, Cu and Cd) by atomic absorption spectrophotometry (ICPS: ARL 3510).
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