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Thirdly, as is shown by results for reference materials, the quality of our metal analyses was very good.
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During two years, water samples for total and dissolved metal analyses were collected every third day at both the inlet and the outlet.
The samples meant for heavy metal analyses were acidified to a pH of 2 using nitric acid.
Water samples for metal analyses were collected into 100 ml plastic bottles and nitric acid (HNO3) added to the samples to bring the pH down to 2 for the metals to remain in solution.
Metal analyses were not reported for preceding VIM-2 preparations.
Samples for metal analyses were digested with concentrated HNO3 in a microwave digestion system.
Cd and Zn metal analyses were performed by ALS Group Czech Republic (Prague, http://www.alsglobal.com/).
Metal analyses were carried out in accredited laboratories of State Enterprise Povodí Moravy (Brno, Czech Republic) using operating procedures according to EN International Organization for Standardization (ISO) standards.
In the subset of children who provided blood samples at both 12 and 24 months (n = 267 vs. total n = 275 for 12-month samples), results from 12-month metal analyses were similar [e.g., mixed-effects model adjusted β, manganese quintile 5 × continuous lead predicting MDI: subset of 267 children, –1.26 (95% CI: –2.17, –0.37); total sample, –1.27 (95% CI: –2.18, –0.37)].
Additional metals analyses were performed by a commercial laboratory.
Samples for trace metals analyses were acidified to a pH <2 after filtration (Appelo and Postma 1999).
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