Sentence examples for metal affinity of from inspiring English sources
We present the Arg160His mutation of Haemophilus influenzae carbonic anhydrase (HICA), which mimics the endogenous metal affinity of Escherichia coli carbonic anhydrase (ECCA).
The study demonstrated that the C-terminal addition of the poly-histidine tag to SjGST increased the metal affinity of the enzyme to the Co-TANOL gel under both native and denaturing conditions and to the Ni-NTA gel under denaturing conditions, whereas the enzymatic activity and antibody-binding ability were not affected.
Although the inhibitor displays an unusual non-metal mediated mode of inhibition, which is an artefact most likely due to the low metal affinity of DXR at the pH used for crystallization, the structural data add valuable information for the rational design of novel DXR inhibitors.
Gene duplication and amino acid substitutions that enhanced the metal affinity of CopA and CopCD proteins may be responsible for the copper resistance found in some Frankia strains.
To test for kinetic effects arising from decreased metal affinity of the mutants compared to WT, mutants R166S, D101A/R166S/D153A/K328A, D101A/D153A, E322Y, D101A/R166S/D153A/E322Y/K328A, and D101A/R166S were also incubated with varying Zn2+ and Mg2+ concentrations for a week.
Measurement of the absolute metal affinities of proteins has been surprisingly challenging, with many erroneous values in the literature [ 10].
Under such a regime the metal affinities of E. coli metal sensors will influence metal occupancy of snail metallothioneins when expressed in E. coli.
Immobilized metal affinity purification of scFv3G1 was carried out using Ni-NTA HisBind Resin that specifically binds the polyhistidine.
In dual expression cultures, metal affinity purification of His6T-GCH1 or His6F-GCH1 revealed that GFRP was able to bind to both forms of GCH1 and could be co-isolated.
Following metal affinity chromatography of TFEα/β, the C92S, C95S and C112S variants rapidly lost the FeS cluster based on the absorbance spectra.
It was initially assumed that free pools of metal ions were available within the cell, such that a nascent polypeptide would acquire its cofactor solely through the metal-affinity of the chelating ligands.
