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Four microbial metabolites were generated by the selected fungal strains, i.e. Macrophomina phaseolina and Fusarium lini (Figures 1 and 2).
Forty-eight additional metabolites were generated by seven taxonomically diverse Basidiomycetes indigenous to agricultural sites, including Agrocybe praecox DSM 13167 from soil (Wetzstein et al. 2006).
Metabolites were generated by incubation of 1.0 mM fatty acids and P450 enzyme (100 pmol) in a 1.0 ml volume of 100 mM potassium phosphate (pH 7.4) for 20 min at 37°C (Gustafsson et al. 2004).
All the metabolites were generated in a NADPH-dependent manner.
Then four different small subnetworks, consisting of genes, reactions and metabolites, were generated corresponding to different tissues.
Theoretical fragmentation patterns and mechanisms of selected metabolites were generated on the basis of chemical structure files using Mass Frontier 4.0 Spectral Interpretation Software (HighChem, Bratislava, Slovakia).
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b The metabolites are generated by RD-Metabolizer and MetaPrint2D-React, respectively.
If the amount of fuel that enters tissue cannot be dealt with, either from the oxidation, or storage, point of view, metabolites are generated which interfere with insulin action, either directly or indirectly [4], [20], [21].
Upon conclusion of Step 1, a draft model with 941 genes, 705 reactions and 708 metabolites was generated.
The fact that both metabolites are generated extracellularly is impossible to reconcile with a role in intracellular signal transduction, and it has therefore been suggested that cADRP functions exogenously in stimulated B cells 6.
In addition to these three models, a space of models differing in either initial concentrations of internal metabolites or fixed concentrations of external metabolites was generated by 2-way sampling of 14 concentrations (8 internal and 6 external), thus yielding a body of 214 = 16,384 individual simulations that is analyzed and discussed separately.
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species were generated
metabolites were synthesized
metabolites were formed
metabolites were found
metabolites were analyzed
metabolites were increased
metabolites were identified
metabolites were excreted
metabolites were targeted
metabolites were entered
metabolites were consumed
metabolites were isolated
metabolites were proved
metabolites were used
metabolites were measured
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