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The metabolites were first characterized by nominal mass measurement of the corresponding protonated molecules.
To determine compounds whose abundance significantly changes in response to an applied condition, the time courses of individual metabolites were first studied to reveal those which significantly either accumulate or dwindle.
These metabolites were first identified in seminal fluid [ 23], but are ubiquitous and highly concentrated growth factors [ 24, 25].
The metabolites were first extracted from 1 mL of urine using semiautomated 96-well plate technology and 30-mg cartridges of a mixed-mode solid-phase extraction sorbent.
Individual metabolites were first normalized to the mean value for the metabolite in one pooled standard for the day the sample was run.
For derivatization, metabolites were first dissolved in 10 µl (2) or 30 µl (7) pyridine containing 40 mg/ml methoxyamine hydrochloride by 90 min incubation at 30°C.
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Species delimitation is an important issue in medicinal fungi, because the production of bioactive secondary metabolites is first species-specific, and then depending on strain characteristics.
In order to perform constraints-based flux analysis, internal metabolites are first balanced under the assumption of pseudo-steady state [ 21, 22].
On the other hand, if the degradation rate of that penultimate metabolite were first order, then that concentration would be a direct function of the concentration of the first metabolite of the pathway, and the regulation would turn into proportional rather than integral control.
The SVS-OH metabolite is first detectable in mouse serum by 10 minutes after gavage administration, and serum levels of SVS-OH continued to be observed by both mass-spectrometric and UV measurements through the last serum samples collected at 50 minutes post-gavage.
Metabolites were at first identified by comparison of their retention time with authentic standards.
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