Dried metabolites were dissolved in 50 μL of H2O before LC-QqQ-MS analysis.
Dried metabolites were dissolved in water and centrifuged at 10,000 rpm for 10 min.
Dried metabolites were dissolved in 20 μL Milli-Q water (EMD Millipore, Darmstadt, Germany) before analysis.
Dried metabolites were dissolved in 20 μL of Milli-Q water before analysis.
Dried polar metabolites were dissolved in 15 μl of 2% methoxyamine hydrochloride in pyridine at 45°C.
The solvent was evaporated completely (SA-Speed Concentrator, H.Saur Laborbedarf) and metabolites were dissolved in 400 μl 100% methanol.
The colored metabolite was dissolved in dimethyl sulfoxide (DMSO).
After a 2 h incubation (37°C, 7% CO2), the plate was centrifuged (500 xg, 5 min), the supernatant removed and the dark-blue metabolite was dissolved in DMSO.
The compounds (monensin A, metabolite 1, and metabolite 2) were dissolved in dimethylsulfoxide (DMSO) and diluted into the medium to give 100, 50, 25, 12.5, 6.3, 3.1, 1.6, 0.8, 0.4, 0.2, 0.1, and 0.05 μg·mL−1, as their final concentrations.
All dry metabolite extracts were dissolved in 800 μL (large samples) or 200 μL (small samples) of D2O containing 1 mM 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic
ADS-I and its metabolites (M1, M2) were dissolved in DMSO at final concentration of 20 mg/mL, and a volume of 50 µL compound in DMSO solution was added to the octanol/phosphate buffer (1 1, v/v) system.
