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Then, the plasma TAC was corrected for metabolites using a sigmoid function derived from the best fit to the measured parent fractions of the arterial samples.
Blood samples were collected at the end of each 2-day period, and plasma was analyzed for AA and other metabolites using a metabolomics approach.
Figure 4a and b shows the extractability of target metabolites using (a) 0%% methanol, (b) 20%% methanol, (c) 40%% methanol and (d) 60%% methanol, at 50 °C on single ion monitoring (SIM) chromatograms.
Jeong-Gu Jeong-Gu Kiml Academy of Agricultural ScieNational Academy of Agricultural Sciencesof a hinh-throughput Suwonn to idescribednd quantheate biocontrol activity of several bacterial isolates andevelopmentive metabofites using a rice leaf and Xoo co-culture technique.
To further characterize dietary effects on metabolism by the reporter algorithm [4], [14], we also determined key metabolites, using a generic genome-scale metabolic model (GSMM) for mouse [15], and key Reactome processes, using curated evidences from the Reactome database [16].
Such extracted feature pairs were then convoluted into feature groups (i.e. metabolites) using a minimum Pearson correlation of 0.9.
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These resultant peak lists were then searched against KEGG [91] and KNApSAcK [43] for secondary metabolites using an in-house developed database search tool (GoBioSpace, Hummel et al., unpublished) while the lipid data was searched against an in-house compiled lipid database (Giavalisco et al., submitted).
The Wadsworth Laboratories measured creatinine in specimens selected for analysis of organophosphate metabolites using an enzymatic reaction on a Roche/Hitachi cobas c 501 analyzer.
To account for variable dilution in urine, the NCEH laboratory measured creatinine in urine specimens selected for analysis of pyrethroid metabolites using an enzymatic reaction on a Roche Hitachi 912 chemistry analyzer (Roche Hitachi, Basel, Switzerland).
The National Wildlife Research Centre provided further analysis of phenolic fractions for possible BDE-209 oxidative metabolites using an Alliance 2695 high-performance liquid chromatograph (Waters Corporation, Milford, MA, USA) connected to a Waters Quattro Ultima triple quadrupole mass spectrometer (LC/MS-MS).
To facilitate interpretation of the results, back-transformation (13) of the loadings generated from the OPLS-DA was performed before generating the loadings plots, which were color-coded with the Pearson linear correlation coefficients of variables (or metabolites) using an in-house developed script for MATLAB (The Mathworks, Natwick, MA).
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