Interestingly, these metabolites generally exhibited type "AC" profile during flux-sum intensification, indicating that the intensification of any essential metabolite in parallel pathways is very detrimental to the cell growth.
Selected metabolites were profiled in parallel with mRNA expression profiling to understand the effect of transcriptional regulatory processes on specific metabolite production that ultimately influence the organoleptic properties of wine.
Metabolites were extracted in parallel from aliquots of all six experimental replicates, three additional replicates from the aforementioned, with a protocol previously described [ 45].
However, it should be noted that this simple interpretation does not account for the complex relationships that may influence the correlation between two metabolites, such as the involvement of metabolites in alternative, often parallel, metabolic pathways.
While our methods allow manipulation of many variables that cannot be controlled in classical biochemical experiments, they do not allow measurements of metabolites and/or other intermediates in parallel.
Although this study was aimed to examining potential dosage regimens for enrofloxacin against a specific bacterial pathogen, the PK parameters of its major metabolite ciprofloxacin was investigated in parallel with the analysis of enrofloxacin.
