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The fungi have been engineered for the desired secondary metabolites by using different laboratory techniques, for example, homologous and heterologous expressions.
The present study aimed to develop a universal methodology for the discovery of biosensors sensitive to particular stresses or metabolites by using a transcriptome analysis, in order to address the need for in vivo biosensors to drive the engineering of microbial cell factories.
Here, we first coordinated the expression of all genes of the mevalonate (MEV) pathway from Saccharomyces cerevisiae using the tunable intergenic regions (TIGRs), and then dynamically regulated the TIGR-mediated MEV pathway to prevent the accumulation of toxic metabolites by using IPP/FPP-responsive promoter.
These results suggested the possibility to obtain fine chemicals including human drug metabolites by using CYP102A1 (Yun et al., 2007) and references therein).
Two-way ANOVA for repeated measures was applied on quantitated metabolites by using a program designed for MatLab (R2008b).
AntiSMASH analysis suggested that P. lilacinum could encode several non-conventional secondary metabolites by using alternate gene clusters.
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The suicide riboswitch was also applied to different metabolite by using artificial allosteric ribozyme.
Level scaling converts the changes in metabolite concentrations into changes relative to the average concentration of the metabolite by using the mean concentration as the scaling factor.
It is used to measure the concentration of a limited number of known metabolites precisely, by using either gas chromatography (GC) or liquid chromatography (LC) coupled to mass spectrometry (MS) or nuclear magnetic resonance spectroscopy (NMR).
Our main emphasis was to unravel the important metabolite genes by using genomic and transcriptomic data despite limited sequencing information.
For metabolite analysis by using HRMAS H NMR, tissues from 10 5-ALA+ve GBMs and 10 5-ALA−ve GBMs were collected in ∼50 mg pieces into 2 ml tubes, instantaneously transferred to dry ice (frozen CO2) and transported to the laboratory where each tube was transferred to liquid nitrogen for storage.
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