The screening of collection of putative stress tolerant plants for PAs and interacting metabolite profiling using GC-MS, LC-MS, and other appropriate tools can be quite useful for gaining knowledge of overall status of metabolites.
Smith, C. A., Want, E. J., O'Maille, G., Abagyan, R. & Siuzdak, G. XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification.
In addition, the effects of exogenous MeJA on other secondary metabolites were evaluated by metabolite profiling using orthogonal signal correction partial least square discriminant analysis (OSC-PLS).
Gene titration studies and metabolite profiling using liquid chromatography mass spectrometry linked the growth inhibition phenotype with the accumulation of the pathway intermediate 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA).
Cognitive function of the mice was tested using a Morris water maze, and pathological changes in the brain tissue were assessed by immunohistochemistry. Global metabolite profiling using ultra performance liquid chromatography mass spectrometry was carried out to identify the metabolites that were differentially expressed in the plasma of mice.
Moreover, for the elucidation of the varieties of mutants that have a small amount of phytic acid considered to be an antinutrient factor in rice grains, several types of low phytic acid-containing mutants (lpa) were analyzed by means of metabolite profiling using GC [33].
For metabolite profiling using LC-MS, 500 mg material was extracted using 5.0 ml 0.1% formic acid (v/v) in 75% aqueous-methanol, as described before [32].
Individual metabolites were analyzed by metabolite profiling using gas chromatography mass spectrometry (GC-MS).
Metabolite profiling using GC-TOF-MS was performed as described in [ 57].
