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We also quantified intracellular NAD+ metabolite levels using LC-MS in strains incubated with 1 mM NA.
Estimated effect of diet on urinary metabolite levels using multivariate models.
Roots were analysed for changes in transcript levels using Affymetrix whole genome DNA microarrays, and for changes in metabolite levels using routine GC-MS based metabolite profiling.
As part of a pilot study to evaluate treatment benefits of cysteamine bitartrate and N-acetylcysteine, we quantitatively measured brain metabolite levels using magnetic resonance spectroscopy (MRS).
For observed or "true" exposure, we calculated 3-month average metabolite levels (using all the nine replicate samples) for each subject and divided the 10 subjects into tertiles.
In many cases, constitutive promoters have been used to drive expression of homologous or heterologous proteins in fungi, with follow-up analysis of metabolite levels using NMR or mass spectrometry approaches (Anasontzis et al. 2014).
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We assessed the significance of the effects of aspirin exposure, gender, and quartile on metabolite level using linear modeling and linear mixed modeling in GenStat 14th edition (VSN International, Hemel Hempstead, UK).
We applied two different methods to help identify sets of SNPs correlated to metabolite levels: one using direct two-way classification based on combination of genotypes at selected loci, and another based on leave-one-out-cross-validation analysis.
Extracellular metabolite levels were used to formulate secretion constraints and differential intracellular metabolites were used to compare and validate the intracellular flux predictions.
Since the relation between exhaled breath metabolites and blood glucose levels shows high inter-person variation, a cross-sectional design may not be ideal for predicting glucose levels using breath metabolites.
Any available network or pathway can be examined at metabolite, reaction, enzyme, pathway or compartment levels using, for example, an interactive graphical user interface [in contrast to static KEGG maps (Kanehisa et al., 2012); Fig. 1b] or information provided at UniProt/SwissProt.
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