As before, we utilized amino acid secretion patterns as constraints to the iMM904 model, sampled the allowable solution space, computed reaction Z-scores for changes from a reference condition (normal potassium and ammonia), and finally summarized the resulting changes using reporter metabolites.
Technically extending the latter metabolic analysis, we characterize tardigrade metabolism using metabolite changes and EST data in the context of metabolic pathways.
Brain metabolite changes will be assessed using single voxel proton magnetic resonance spectroscopic (MRS) imaging (TR = 1500 ms, TE = 30 ms, and 64 averages).
MetaMap Tox-based pattern ranking using metabolite changes induced by the high dose lisinopril treatment identified matches with validated patterns associated with kidney toxicity (ACE inhibitor like) and platelet aggregation inhibition (Supplemental Figure 7).
On the other hand, one may easily highlight in a whole metabolic pathway map those metabolites whose concentration were detected as having changed using the YeastCyc database.
MetaMap Tox-based pattern ranking using the metabolite changes induced by the high dose captopril treatment matches with validated patterns associated with kidney toxicity, such as glomerular-tubular defect (Supplemental Figure 5).
Significant metabolite changes were mapped to metabolic pathways using MapMan [ 36] and all identified compounds presenting significant changes in leaves and roots to different nitrate treatments are listed in Additional files 7 and 8.
Furthermore, we aim to provide additional information about the nature of anatomical changes by exploring changes in metabolite concentrations using magnetic resonance spectroscopy (MRS) and white matter integrity using diffusion tensor imaging (DTI).
Using PROFILE clustering, the detected metabolite changes were analyzed in the context of the underlying metabolic pathways.
Metabolite changes induced in the seedlings under the different conditions were analyzed using GC-MS (Table 1, Additional file 1: Table S1).
