In contrast, housekeeping activities such as amino acid and lipid metabolism were underrepresented in the metatranscriptome.
However, a recent 3× coverage genome sequence survey of a human isolate of Enterocytozoon bieneusi revealed that genes relating to energy metabolism were underrepresented in general (Akiyoshi et al. 2009).
Although CDSs involved in intermediary metabolism were underrepresented among the deleted genes, 21 (42%) of deleted CDSs of this category were dehydrogenases (such as acyl-CoA short-chain alcohol, saccharopine, and aldehyde dehydrogenases), which are central enzymes in anaerobic metabolism (22 ) and important for survival in poorly oxygenated environments such as soil (23 ).
In contrast, amino acid metabolism was underrepresented in the IBD-PRE group (Fig. 4B ).
Proteins that function in energy metabolism are underrepresented only in the case of ORB+ gene sets (fig. 3 A ), whereas those engaged in DNA metabolism, central intermediary metabolism, and transcription are underrepresented only for ORB− sets (fig. 3 B ).
However, genes identified in "amino acid metabolism" pathways were underrepresented.
In contrast categories "Translation, ribosomal structure and biogenesis" (J) and "Nucleotide transport and metabolism" (F) were underrepresented in A. muciniphila specific genes.
In contrast, genes involved in intermediary metabolism, information pathways, and cell wall/cell processes were underrepresented among the deleted CDSs.
Major metabolic pathways such as photosynthesis, central carbon metabolism, energy metabolism as well as lipid, amino acid, and protein biosynthesis were overrepresented whereas stress, regulation of transcription, and signaling categories were underrepresented.
"But, we still found that women were underrepresented".
Other counties, especially those in suburban areas, were underrepresented.
