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In addition, mathematical models and molecular methods can be used to elucidate metabolically engineered systems and to identify targets for performance improvement.
In comparison, the latter bioproduction strategy highlighted by a limited number of metabolically engineered systems is undoubtedly 'greener' [ 5- 10].
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FBrAtio was used to model wild-type metabolism and metabolically engineered strains of C. acetobutylicum where only flux ratio constraints and thermodynamic reversibility of reactions were required.
To our knowledge, this study represents the first report of a S. pombe strain that was metabolically engineered using a CRISPR-Cas9 system, and demonstrates the possibility of engineering S. pombe for the production of value-added chemicals.
Thus, this study demonstrates that the baculovirus-insect cell expression system can be metabolically engineered for N-glycoprotein sialylation by the addition of two mammalian glycosyltransferase genes.
Here, we constructed metabolically engineered S. pombe strains using a CRISPR-Cas9 system and also demonstrated D-lactic acid (D-LA) production from glucose and cellobiose.
Thus, metabolically engineered yeast P. pastoris represents a valuable, whole-cell system for high-level production of -nootkatone from simple carbon sources.
It has been reported a two-phase culture system with dodecane for retinoids extraction using a metabolically engineered E. coli (Jang et al. 2011).
Results from this cell-free system were confirmed via the generation and analysis of metabolically engineered strains of E. coli.
In this study, semi-continuous lycopene overproduction and in situ extraction using a metabolically engineered E. coli strain is attained for the first time with an octane-aqueous culture system (1 5 volume ratio).
For several years, we and others have suggested that transgenic oilseeds metabolically engineered with the heterologous capacity to synthesize omega-3 LC-PUFAs represent a novel (terrestrial) production system, which could substitute for some or all of the applications currently using fish oils.
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