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Studies of prokaryotic life in the Discovery, L'Atalante, Urania, and Bannock basins using epifluorescence microscopy, analyses of 16S ribosomal RNA (16S rRNA) gene sequences, and measurement of sulfate reduction, methanogenesis, and heterotrophic activity have revealed metabolically active bacterial and archaeal communities [217] [222].
Prior to inoculation for experiments, a loopful of culture from LB agar plate was inoculated in 10 ml LB broth in tube and incubated overnight at 37 °C/150 rpm to obtain log-phase metabolically active bacterial cells.
Moreover, the green intracellular (vital) Fluorescein staining originates only in metabolically active (bacterial) cells.
On the other hand, the spiking of N into the sediments induced changes in the metabolically active bacterial community structure.
Combined, MICRO CARD FISH and DNA and RNA clone libraries, however, allowed for accurately quantifying different bacterial groups and their activity as well as a detailed phylogenetic insight into the fractions of present versus metabolically active bacterial groups.
SLOT enables the visualization of living biofilms in 3D by detecting the wavelength-dependent absorption of non-fluorescent stains like e.g. reduced triphenyltetrazolium chloride (TTC) accumulated within metabolically active bacterial cells.
Similar(53)
LAM antigen is a lipopolysaccharide present in mycobacterial cell walls which is released from metabolically active or degenerating bacterial cells and appears to be present only in people with active TB.
This may indicate a metabolically active Gram-negative bacterial population in comparison to a dormant spore-forming population.
Reactive arthritis (ReA) is characterized by the presence of metabolically active bacteria or bacterial macromolecules in the synovial compartment but microbial pathogens cannot be cultured from the synovial material.
The role of the cas2 gene in the acquisition of resistance to new phages might be of particular importance in a metabolically active and proliferating bacterial community, such as the rhizosphere microbiota (Ofek et al., 2014), which represents an ideal substrate for bacteriophage infections.
The higher amount of rRNA in active than in dormant cells associated with the higher number of ribosome in active cells provides a tool to determine the metabolically active members of the bacterial community (Poulsen et al., 1993).
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