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Ethanol metabolic yield (based on hydrolyzed glucan and xylan) and ethanol yield (based on glucan and xylan in untreated CS) were 77 and 57 %, respectively.
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Metabolic yield calculated based on the theoretical ethanol yield from consumed glucose and xylose, 0.51 g ethanol/g sugar.
Yield based on polymer weight, 73.1%.
Ethanol metabolic yield was calculated based on the theoretical ethanol yield from consumed glucose and xylose, which is 0.51 g ethanol/g glucose or xylose.
Ethanol metabolic yield was calculated from consumed glucose and xylose during fermentation, based on the theoretical ethanol yield (0.51 g ethanol per g glucose/xylose).
Based on the glucan content, AFEX pretreatment efficiency and metabolic yield of ethanol fermentation, the calculated yield of ethanol from AFEX-treated CS was 69.3 gallon/ton of biomass, which was 1.5-fold higher than that of untreated silage (45.5 gallon/ton).
bMonomeric sugar conversion was calculated based on the ethanol concentration and the remaining monomeric glucose and xylose in the fermentation broth after 168 h with the assumption of ethanol metabolic yield the same as SHF.
This method also provides a direct, simple method to estimate the metabolic yield of a colony.
The ethanol metabolic yield is used to determine fermentation efficiency; fermentation with all sugars converted to ethanol would have a metabolic yield of 1.
AFEX was able to produce high fermentation metabolic yield (98%) even without external nutrient supplementation.
Carbon source (glucose or xylose) did not significantly affect the metabolic yield in KO11 fermentation.
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