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Analyzing the metabolome means to make a metabolic snapshot of the cell, which is challenging because metabolism has turnover rates in the range of seconds.
A drive to increase the speed and width of metabolic analysis through the development of metabolite profiling procedures based upon GC/MS has enhanced the metabolic snapshot that can be quickly taken and in all applications so far reported has led to substantial surprises.
Although this steady-state metabolomics is a metabolic snapshot supporting our predictions, inferences about metabolic fluxes are necessarily limited.
Although these data reflect a metabolic snapshot of a dynamic process, they are consistent with our additional findings.
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Fig. 2A shows the metabolic snapshots of the glycolysis pathway indicating enhanced glycolytic activity as an initial response to kainite treatment; most of the glycolytic intermediates were significantly decreased 30 min after kainate administration (blue-colored graphs), suggesting the occurrence of acute glycolysis.
The 2 mutants showed distinct metabolic snapshots that were consistent within each biological replicate of mutant plants.
Metabolomics allows a relatively complete snapshot of metabolic processes in a sample using different mass spectrometry methods.
Lipidomics, especially global/untargeted lipidomics, has the power to reveal a broad snapshot of metabolic events occurring in both closely and distantly related pathways.
The ability of simultaneously obtaining an almost complete snapshot of metabolic activity in a given system is a strength of metabolomics, with the caveat that some metabolites may potentially require special processing methods, are not stable, or that markers of interest are proteins.
Our metabolic profiling provides a snapshot of the complex human metabolome.
The method provides a quick snapshot of metabolic profiles of lead compounds for the CXCR3, including their bioaffinity, and thereby facilitates this type of profiling earlier on in drug discovery, to be used for larger sets of interesting compounds in a drug discovery pipeline.
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