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It is possible that a metabolic shift also contributes to the extended lifespan of certain PHB-depleted C. elegans mutants.
This metabolic shift also occurs in T. kodakarensis[ 34, 35] and, based on the numbers of cDNA reads obtained from RNA preparations isolated from T. kodakarensis cells growing in pyruvate medium before and after sulfur addition, the transcription regulation is also conserved in T. kodakarensis.
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Therefore it is likely that such a metabolic shift was also involved in our fermentation tests.
In addition, this metabolic shift may also serve a neuroprotective role by counteracting oxidative stress in the brain.
This metabolic shift has also been observed in dauer larvae and long-lived daf-2 mutants [ 55, 56].
Metabolic shifts are also observed in the pressure-loaded RV.
We found that SDH activity was increased following BMP2 treatment, suggesting that pro-differentiating agents, such as BMPs, may promote a metabolic shift toward oxidative phosphorylation also in tumor cells.
We would like to emphasize that subsequent to the fortuitous metabolic shift, additional mechanisms can also come into play to stabilize the aerobic glycolysis.
A metabolic shift towards secondary metabolites was also observed under P starvation conditions, but in contrast to low N stress, amino acids accumulated under low P conditions while phosphorylated intermediates decreased.
Changing the medium pH may also induce a metabolic shift (Zhu and Yang 2004).
Complete oxidation of glucose in the TCA cycle (EM 43) is also selected, reflecting the metabolic shift induced during glucose fed-batch experiments.
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