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Metabolite and Metabolic Scheme.
Figure 3 Metabolic scheme for PRA in G. striatum.
The information of each ingredient was divided into three categories: identification label, metabolic scheme and bioactivity information.
The database HIM comprises three data fields for each active ingredient: identification label, metabolic scheme and bioactivity information.
We demonstrate that traditional expensive energy regeneration systems, such as creatine phosphate, phosphoenolpyruvate or phosphoglycerate, can be replaced by a cost-effective metabolic scheme suitable for cell-free protein synthesis applications.
A crucial feature in the adaptation of any bacterium to alternative energy sources and changing environmental parameters is the balance of oxidative and reductive reactions in the metabolic scheme that are dependent on electron donor and acceptor availability.
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Inactivation of competing reactions and manipulation of culture conditions has lead to higher hydrogen yields, near those predicted by metabolic schemes.
As a result, metabolic schemes could be constructed, including bioaffinity information per metabolite.
That simplifies modeling considerably: to model any mixture, it is enough to draw the individual metabolic schemes of its constituents and write their joint equation system.
The software Isodyn (13) simulates the dynamics of isotopic isomer distribution in central metabolic pathways, and is further enhanced by algorithms facilitating the transition between various analysed metabolic schemes as well as tools for model discrimination.
The software Isodyn, which simulates the dynamics of isotopic isomer distribution in central metabolic pathways, was supplemented by algorithms facilitating the transition between various analyzed metabolic schemes, and by the tools for model discrimination.
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