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Over 10% of the genes with the catalytic activity annotation that are upregulated in III M male head are predicted to encode proteins involved in a metabolic process, including metabolism of organic acids, amino acids, and lipids.
Genes related to inflammation, lipid metabolism process and another metabolic process (including carbohydrate protein and nucleic acid metabolism) and hematological system developments were selected [ 12, 13].
Negative genetic interactions were enriched for the following Gene Ontology categories (supplementary material Table S6): Transcription, RNA Metabolic Process, including genes for tRNA processing, and Signal Transducer Activity/Signal Transduction.
Gene Ontology analysis of the differential proteins showed high level of enrichment in the nodes belonging to metabolic process including glucosinolate biosynthesis and response to stimulus.
Many of these genes code for proteins involved in the lipid metabolic process, including bile secretion, adipocytokine signalling pathway, biosynthesis of unsaturated fatty acids, linoleic acid metabolism, fatty acid elongation and fatty acid biosynthesis, and fat digestion and absorption.
In effect, the variations of pH are considered as indicators of change in the metabolic process, including enzymes (Sandhya et al. 2005).
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Also, the sulfur metabolic process includes genes involved in sulfur assimilation, a biochemical process that consumes reducing equivalents.
The GO term "metabolic process" included 242 differentially expressed genes, a number of which have been measured by RT-qPCR, including UCP1, CYC1 and CS.
For example, CVGs in metabolic process include enzymes from various biochemical pathways (Additional file 3), which may indirectly affect the vascular development.
Also, a variety of terms related to metabolic processes, including carbohydrate metabolism, protein metabolism, or secondary metabolism, were over-represented in gene families.
Our analysis confirms that methapyrilene-mediated hepatotoxicity is accompanied by induction of genes involved in protein translation, structural remodeling, cell death/apoptosis, and the repression of several metabolic processes including lipid metabolism, steroid metabolism, and fatty acid β-oxidation.
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