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Proteins of the metabolic process group with altered protein expression are involved in the citric acid cycle, gluconeogenesis, glycolysis and other important pathways.
In the "Cellular carbohydrate metabolic process" group, genes affecting cell wall biosynthesis such as 1,3-β-glucan synthases, cellulose synthases and putative glycosyl transferases 8 were present.
We identified twenty differentially regulated genes belonging to the carbohydrate metabolic process group, eight that are up-regulated in MYC fruit and twelve that are down-regulated (Table 4).
Therefore, we hypothesized that the 18,292 unigenes classified into the "metabolic process" group and the 15,272 unigenes classified into the "catalytic activity" group might serve as good candidates for the identification of novel genes that participated in the SG biosynthesis and accumulation pathways.
In our study, we identified eight up-regulated genes belonging to the carbohydrate metabolic process group, seven out of them specifically induced after pathogenic inoculation at 72 hpi, and one cell wall invertase (CWI) (Glyma.15G024600) induced by the same isolate at both time points.
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Among those sequences that could be assigned a functional classification, the largest categories were catalytic activity and metabolic processes, groups that include the majority of cellular enzymes and components of amino acid, carbohydrate and lipid metabolism.
Within the GSR dataset, 29% of the sequences were annotated using the Arabidopsis Gene Ontology (GO) with the largest categories of assigned function being catalytic activity and metabolic processes, groups that include the majority of cellular enzymes and components of amino acid, carbohydrate and lipid metabolism.
Only the Pyridoxine metabolic process functional group was downregulated with regards to non-virulent strains at time point 30 min. This group includes genes (SNZ, SNO2) involved in pyridoxine metabolism and thiamine biosynthesis.
Here, the conserved domains and GO annotations analysis showed that OsSTA192 (OsNek3) and OsSTA202 (OsPDC3) had the kinase activity, and OsSTA28, OsPDC3 and OsNek3 were included in metabolic process genes group.
The fundamental processes concluded from GO analysis also include developmental process (8 CVG groups), metabolic process (12 CVG groups), transport (8 CVG groups), and response to abiotic or biotic stimulus (8 CVG groups).
Relative GO category enrichment in the slow group included primary metabolic process, nitrogen metabolism and macromolecular processes plus several genes important for iron uptake and utilization.
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