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One common example is that microarray datasets use gene probe IDs but metabolic pathways use gene locus names.
This study used a predefined set of chemical substructures (KCF-S); however, it is known that some metabolic pathways use their characteristic chemical substructures.
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To test how gene duplication and associated gene expression variation may shape Arabidopsis metabolism, I focused on transcripts associated with specific metabolic pathways using the AraCyc databases predictions [20], [21].
Knowledge of the metabolic pathways used by anaerobic bacteria to break down hydrocarbons has allowed us to identify unique intermediate compounds that can be used as biomarkers for in situ activity.
Analysis of the Sulfobacillus genomes illuminates metabolic pathways used in various transformations of carbon, nitrogen, and sulfur metabolism (summarized Figure 5 and Additional file 5: Table S2).
Out of these, 197 genes could be associated with different metabolic pathways using the RiceCYC 1.2.
MapMan analysis of the secondary metabolic pathways using the differentially expressed genes derived from (a) comparison C + F vs C, and (b) comparison W + F vs W. Red arrows indicate the pathways enriched in up-regulated genes.
MapMan software (Thimm et al. 2004) was used to visualize the distribution of DEGs into different bins and their appearance in different metabolic pathways using Oryza - specific mapping file.
Shown in Table 2. is the distribution of the unigenes into general metabolic pathways used by KEGG.
It also implies that there is potential for discovery of new genes in this organism and possibly of new gene networks and metabolic pathways using the assembled data.
Of the discriminating masses 13.3%, 9.5% and 9.3% for ICD, CCD and healthy groups, respectively, could be assigned to metabolic pathways using MassTRIX.
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