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Applying pulsed SILAC and GC-MS based stable isotope resolved metabolomics [ 21] we identified the major metabolic pathways altered in the early response of Chlamydomonas to salt stress.
Our results demonstrate the effectiveness of combining CE MS metabolite data and transcript data to identify a subset of metabolic pathways altered by this mutation and confirm the applicability of this approach to chemically complex small-volume measurements.
In the past, much attention was spend on the composition of membrane lipids during cold adaptation and heat-shocks, but only few studies investigated transcriptomic changes to identify general metabolic pathways altered during temperature adaptation processes.
Rimonabant-induced weight loss occurs through central effects in reducing food intake and through peripheral metabolic pathways (altered substrate utilization, changes in adiponectin, and changes in energy expenditure) (13).
In addition to quantitatively defining the specific metabolic pathways altered by experimental manipulation, our results suggest that the GSVD algorithm can identify discrete series of metabolic reactions altered by experimental manipulation.
Prediction of metabolic pathways altered in the different strains by treatment with H2O2 or due to the mutation of arcA was performed using the software Gene Set Enrichment Analysis (GSEA) [ 34], with the KEGG database for S. Typhimurium LT2 as a reference.
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In this study we focused on the hypothesis that uteroplacental insufficiency may affect skeletal muscle metabolic pathways altering the expression of insulin receptor and of key enzymes of intramuscular lipid metabolism.
Application of an Active Modules algorithm to transcriptomic data identified key metabolic reactions and pathways altered in response to nitrogen stress, which are central to survival under nitrogen limiting environments.
Gene ontology (GO) and KEGG pathway analyses indicated seven reliable metabolic pathways are altered in the mutant, including carbon metabolism, starch and sucrose metabolism and biosynthesis of amino acids.
Of the metabolic pathways with altered expressed genes, 25% were involved in amino acid metabolism.
Supplementary Table S1 List of rice metabolic pathways significantly altered by cellulase treatment (DOC 44 kb) Supplementary Table S2 List of primers used for real time PCR analysis (DOC 47 kb) Supplementary Figure S1 HR like reaction upon infiltration with ClsA.
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