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Second, we evaluated the metabolic path of sucrose, including the extent of sucrose hydrolysis, during this radial transfer within a growing culm (axillary branch).
Previous studies to determine the metabolic path of sucrose have often involved application of the tracer sucrose to slices of culm tissue (stem disks).
These results suggest that isotope discrimination during radial transfer of sucrose had little influence on the redistribution of H-label between the hexose moieties of sucrose as an indicator of the metabolic path of the sucrose.
The results of our study and previous findings led us to assume that the major metabolic path of inhaled NO is as follows: inhaled NO reacts with hemoglobin, forming nitrosyl-hemoglobin (NOHb), and from NOHb, nitrite (NO2-) and nitrate (NO3-) are generated.
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Since all cellular life possesses the core set of pathways we're interested in reconstructing, a comparison of the metabolic paths of extant organisms would only reflect differences in metabolic regulation rather than differences in structure.
The expression patterns of sugar transporters in sugarcane culm do not exclude any of the suggested cellular or metabolic paths of sucrose during radial transfer.
The cellular and metabolic paths of sucrose during radial transfer between phloem and the intracellular compartment in growing sorghum internodes have not been studied in intact plants, but would be expected to be symplasmic and without extensive sucrose hydrolysis based on comparison with other growing plant tissues [ 18].
It is located near putative genes involved in glycolysis/glyconeogenesis (e.g., phosphoglycerate mutase and phosphoenolpyruvate synthase), thus generating a bidirectional metabolic path for the utilization/generation of glycogen.
We consider a metabolic path to be an optimal one, if y is minimum and the c i -values of all the enzymes catalyzing the reactions in that path are greater than zero.
Network redundancy increases through expanded inclusion of isoenzymes, which introduce parallel metabolic paths for the production of chemical intermediates or products.
When two quantified genes are co-regulated, e.g. when they are part of the same metabolic path, the similarity of expression patterns can incorrectly point to high stability under the experimental setup.
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