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Genome scale reconstruction of a metabolic network involves thousands of metabolites and reactions.
Metabolic network involves some sequential reactions whose chemical transformation patterns are conserved, and these conserved sequences are referred to as 'reaction modules' (Muto et al., 2013).
Although metabolic network involves some sequential reactions whose chemical transformation patterns are conserved (Muto et al., 2013), these conserved patterns have not been considered.
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These can potentially also be used to predict optimal pathways in a metabolic network, involving the minimum number of bond changes.
In this work, an approach was developed to provide an online estimation of the intracellular flux distribution of cells grown in perfusion cultures, based on a comprehensive metabolic network involving 40 biochemical reactions and 46 components.
In this work, an approach was developed to provide an on-line estimation of the intracellular flux distribution of cells grown in perfusion cultures, based on a comprehensive metabolic network involving 40 biochemical reactions and 46 components.
In particular, studies on the endocrinology of exercise and training have demonstrated the existence of an integrated metabolic network involved in regulating hormones and cytokines [ 11].
Using this strategy, we were able to identify the metabolic network involved in cysteine, methionine, serine, glycine, glutathione, and SAM synthesis.
The typical workflow for closing gaps in the rubber metabolic network involved downloading protein sequences that could perform the missing enzymatic activity either from Arabidopsis or other plant or bacterial genome and then using it as query for TBLASTN.
Many of the enzymes in clusters of this region, such as cytochrome P450s, glycoside hydrolases and tyrosinases are at the edge of the metabolic network involved in synthesis of the final secreted metabolites or degradation of the external sources of carbon.
AMBIENT identified 20 candidate up- and down-regulated active modules in the metabolic network, involving a total of 330 enzyme-encoding genes (out of 963 genes included in the model) that showed a co-ordinated response to nitrogen limitation (Table 1).
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