The completeness of, and confidence in, our understanding of culture metabolism has permitted the development of metabolic models for recombinant cell lines that will facilitate enhancement of bioprocess performance.
FAME [ 22] is a web-based modeling tool for creating, editing and analyzing metabolic models for microorganisms from the KEGG database.
Fast automated reconstruction of genome-scale metabolic models for microbial species and communities.
Wang, Y., Eddy, J. A. & Price, N. D. Reconstruction of genome-scale metabolic models for 126 human tissues using mCADRE.
More than a decade ago, the first genome-scale metabolic models for two of the most relevant microbes for biotechnology applications, Escherichia coli and Saccaromyces cerevisiae, were published.
The isoform specific subcellular localization determined here suggests that peroxisomal microbodies should be considered in the design of metabolic models for carbon assimilation in C. reinhardtii.
This raises the question of whether ecosystem-level behavior of structured communities can be predicted using genome-scale metabolic models for multiple organisms.
Recently, a number of methods and tools have been proposed to allow the use of genome-scale metabolic models for the phenotype simulation and optimization of microbial strains, within the field of Metabolic Engineering (ME).
The intrinsic differences within the metabolic characteristics of the 'Candidatus Accumulibacter phosphatis' clades (Carvalho et al. 2007; Flowers et al. 2009; Slater et al. 2010), urges the need to study these metabolic differences and develop clade specific metabolic models for better understanding and description of EBPR processes.
The comparison of the newly obtained genome-scale metabolic models for L. reuteri ATCC PTA 6475 and ATCC 55730, along with the visualization of experimental data was carried out within the SimPheny™ software platform (Genomatica, Inc., San Diego, CA).
Establishment of in silico metabolic models for strain engineering [ 55,56,57 ].
