Extracellular metabolites are defined as in the "metabolic footprinting" medium [ 16] and reproduced in Table 3 for completeness.
Values are as defined in the "metabolic footprinting" medium [ 16]. 151 elasticities are calculated from models within BioModels, using symbolic differentiation.
Further work is needed to assess the total metabolic variance over medium- and long-term studies.
These metabolic engineering and medium optimization approaches may be helpful for improving 1-propanol production by the C. glutamicum strains described in this study.
During storage, RBC and their preservative medium suffer metabolic, biochemical, and molecular changes commonly referred to as "storage lesion" (Figure 1; Table 1)[10, 31].
In this work, metabolic engineering methodologies including medium component analysis, 13C-labeling experiments, and computer-aided simulation analysis were applied to characterize the metabolic phenotype of soft tissue sarcoma cells derived from p53-null mice.
After metabolic labeling, the radioactive medium was removed and cells were washed twice with PBS.
Thus, the initially simple environment becomes a mixed-substrate growth medium where metabolic interactions can take place.
The oxidized, blue, non-fluorescent resazurin is reduced to a pink fluorescent dye in the medium by metabolic activity.
For a metabolic perturbation experiment, RM28 medium supplemented with 10 mM L-lysine and 1 mM L-threonine [ 35] was used at the third medium change.
Infection was performed using a highly concentrated seed virus solution (modified protocols) to keep metabolic influences of spent medium of virus seed as low as possible.
