Sentence examples for metabolic labeling using from inspiring English sources

It was previously shown that K8 and K19 are phosphorylated on tyrosine residues after treatment with the potent and irreversible tyrosine phosphatase inhibitor pervanadate, based on metabolic labeling using 32PO4 and phosphoamino acid analysis, as well as anti-phosphotyrosine antibodies [21].

This result is consistent with a recent report of metabolic labeling using an alkynyl derivative of fucose.

We recently published the first quantitative study of the secretome of primary human articular chondrocytes (HACs) by chondrocyte metabolic labeling, using an in vitro model of inflammation by stimulation with IL-1β [ 12].

For metabolic labeling using SILAC, cells were grown in medium containing either only 'light' arginine or only 'heavy' 13C6-arginine, resulting in incorporation of labeled arginine in all sites.

For metabolic labeling using SILAC, GM00637 cells were cultured in DMEM media for SILAC (DMEM media minus l-lysine and l-arginine, Thermo Scientific, Waltham, MA), supplemented with light lysine and arginine, or heavy stable isotope-labeled [C6,N2]- l-lysine and [C6]- l-arginine, following previously published procedures.

O-GlcNAc metabolic labeling was performed using Click-iT GlcNAz metabolic glycoprotein labeling reagent (tetraacetylated N-azidoacetylgalactosamine and glucosamine) from Invitrogen, USA.

Metabolic labeling of proteins using stable isotope labeling with amino acids in cell culture (SILAC) [ 17] has become a powerful technique for quantitative MS-based proteomics [ 18].

Metabolic labeling is routinely used in simple systems, such as yeast and cultured mammalian cells and has even been applied to simple organisms, including C. elegans and D. melanogaster, to quantify hundreds to thousands of unmodified and phosphorylated peptides [19], [20].

The in vivo labeling used in this study has been previously limited to myocardial metabolic functioning, tumor proliferation, and select inflammatory processes.

There are three primary experimental approaches for using metabolic labeling to compare the steady-state levels of protein abundance on a proteomic scale: (1) full metabolic labeling, (2) stable isotopic labeling by amino acids in cell culture (SILAC), and (3) partial metabolic labeling.

The first large-scale comparative study on the effects of treatment with the antimalarial drugs artemisinin and chloroquine on the proteome of P. falciparum was performed using metabolic labeling followed by identification and quantification with MudPIT LC-MS.