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The cell-selective metabolic labeling strategies have emerged from protein and glycan labeling.
Metabolic labeling of cells expressing the siRNA to GRWD1 shows a decrease in global protein synthesis.
This technology involves metabolic labeling of glycans with a specifically reactive, abiotic functional group, the azide.
Several strategies exist for the metabolic labeling for the relative quantification of proteins, depending on the experimental system of interest.
The SILIS was produced through metabolic labeling where 15N was uniformly introduced at every nitrogen atom in the studied proteins.
Herein, we review the recent methodological developments aiming to endow metabolic labeling strategies with cell-type selectivity.
Metabolic labeling, followed by cold chase revealed little difference in stability of proteins expressed from mH5 or pSyn promoter constructs.
There are three primary experimental approaches for using metabolic labeling to compare the steady-state levels of protein abundance on a proteomic scale: (1) full metabolic labeling, (2) stable isotopic labeling by amino acids in cell culture (SILAC), and (3) partial metabolic labeling.
This was termed "metabolic labeling".
Metabolic labeling assay confirmed that 17βE2 accelerates MS-KIF18A turnover.
Compactin was confirmed to inhibit cholesterol synthesis by metabolic labeling.
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