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In summary, we showed that BLR1, BLR2 and ENV1 exert important metabolic functions, not only in light, but also in darkness.
The arrest response to ART in P. falciparum rings and trophozoites, perhaps acting as a trigger to prevent the parasite from maturing, implies that key metabolic functions (not yet identified) may be disrupted by ART.
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Despite the prominent metabolic phenotype in Ob mice, metabolic functions are not represented in these ontologies.
As indicated by our toxicity model (figure 1), mercury may adversely affect learning if either metallothionein and glutathione metabolic functions are not functioning properly and supported by key dietary nutrients.
Interestingly, the analysis indicated that the initiation of transcription of genes encoding some key metabolic functions was not restricted to the light or to the dark exclusively, as in Cyanothece 51142 [ 8, 21].
It is likely that the gene products of proV have other essential functions that may or may not be related to metabolic functions and were not accounted for in the current network description.
In particular, the use of metabolic networks from selected organisms as references does not allow the identification of metabolic functions that are not present in these templates.
While clade A and B-associated cooS genes are likely used for acetogenesis, our analyses suggest that cooS genes associated with clade C may encode CODH enzymes used for metabolic functions that are not directly associated with this metabolism.
Their potential to discover novel metabolic functions has however not been demonstrated.
Metabolic reconstructions can give a valuable contribution to functional genomics, by uncovering missing metabolic functions (i.e., functions not assigned to genes) and hence putting forward the identification of the corresponding genes [ 16, 17].
Gene ontology (GO) analysis of genes whose expression was altered in ObC as compared with LnC obtained a large set of ontologies, within which metabolic functions were present but not prominent (Fig. 3B).
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