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Recently, C. glutamicum was shown to have increased metabolic flow through the glycolytic pathway and reductive branch of the tricarboxylic acid (TCA) cycle under oxygen-deprived conditions, resulting in the exclusive production of the organic acids lactate, succinate, and acetate (Inui et al. 2004).
Furthermore, to enhance metabolic flow through the phenylpropanoid pathway and maintain a high basal concentration of sinapyl alcohol in the leaves, UGT72E3/2 was combined with the sinapyl alcohol synthesis pathway gene F5H encoding ferulate 5-hydroxylase and the lignin biosynthesis transcriptional activator MYB58.
A complex interplay of regulators controls the metabolic flow through the aromatic, Asp and BCAA-pathways, which includes feedback inhibitors of regulatory enzymes [ 31, 32].
Interestingly, these studies show that ROS-dependent inactivation of pyruvate kinase or a switch in isoform expression can redirect metabolic flow through the pentose phosphate pathway, which makes the reduced nicotinamide adenine dinucleotide phosphate (NADPH) required to maintain cellular redox homeostasis.
Each of the predicted enzymes for the downstream half of the MEP/DOXP pathway (IspF and onwards) are encoded by single nonredundant curated contigs with high to very high sequence coverage in the B. braunii Showa transcriptome (200.7, 242.9, and 426.6 for IspF, IspG and IspH, respectively), indicating vigorous transcription and perhaps robust metabolic flow through these enzymes.
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These results suggested that the supplementation of lysine and threonine influenced X261 and X333 in a delayed fashion due to metabolic flow passing through various reactions in long and complicated pathways (see Figure 4).
The volume of CO2 (V ˙ CO 2 ) produced by D. melanogaster larvae is a good measure of aerobic metabolic rate, and flow-through respirometry allows dynamic, high-resolution quantification of metabolic CO2 production [ 32].
CLSM also has the advantage of allowing real-time imaging of localized processes of attachment, growth and dispersal as well as metabolic activity and flow through the biofilm.
Before diving, the sea lion stayed in the dome to measure its metabolic rate at the surface using flow-through respirometry (Fahlman et al., 2008a).
We measured metabolic rate as CO2 production using a flow-through respirometry system (Qubit System Research, Kingston, Ontario, Canada) and a modification of the method described in [76].
Rate of CO2 emission was used as a proxy for metabolic rate and was measured using standard flow-through respirometry techniques according to the methodology described in (Schimpf et al., 2012).
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