Here, we discuss a new conceptual framework, termed "precision metabolic engineering," involving the design and engineering of systems that make different products in response to different signals.
Furthermore, there are other examples of metabolic engineering involving deleting iclR to increase productions of compounds.
With a relatively short-life cycle, complex metabolic engineering involving the stacking of numerous pathway genes is therefore feasible in Camelina [ 2 ], as described below for LC-PUFA engineering.
In addition, the strategies to increase lipids accumulation via metabolic engineering technology, involving the enhancement of fatty acid synthesis approach, the enhancement of TAG synthesis approach, the regulation of related TAG biosynthesis bypass approaches, the blocking of competing pathways and the multi-gene approach, are discussed in detail.
Some of the most productive metabolic engineering strategies involve genetic modifications that cause severe metabolic burden on the host cell.
The metabolic engineering approaches involved introduction of additional copies of transcription factor SEF1 and IMH3 (coding for IMP dehydrogenase) orthologs from Debaryomyces hansenii, and the homologous genes RIB1 and RIB7, encoding GTP cyclohydrolase II and riboflavin synthetase, the first and the last enzymes of riboflavin biosynthesis pathway, respectively.
These knockout strategies were generated using GDLS (Genetic Design through Local Search) [ 10], an efficient heuristic for generating metabolic engineering strategies involving multiple knockouts from genome-scale models, extending the capabilities of the computationally-expensive optimal search proposed by OptKnock [ 9].
It is expected that the intron donor plasmid and associated gene disruption methodologies detailed herein will add to the expanding genetic toolkit available for C. pasteurianum and lead to rewarding metabolic engineering efforts involving this important biotechnological bacterium.
We present a detailed analysis of the impact of perturbations in redox cofactors in the cytosol or mitochondria on glucose and energy metabolism in Saccharomyces cerevisiae to aid metabolic engineering decisions that involve cofactor engineering.
