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At the end, the perspectives of systems metabolic engineering based on synthetic biology are discussed, with selected exemplary case studies.
Inverse metabolic engineering based on elementary mode analysis was applied to maximize the biomass yield of Escherchia coli MG1655.
Rational genome-wide metabolic engineering based on systems and synthetic biology tools, which is termed 'systems metabolic engineering', is rising as an alternative to overcome these problems.
Metabolic engineering based on a comprehensive understanding of the complex biological systems supported by systems biology, or systems metabolic engineering, has enabled the engineering of microorganisms into microbial factories by thoroughly redesigning and reconstructing their metabolic and regulatory networks.
Further inverse metabolic engineering based on functional complementation suggested that efficient xylose assimilation is attributed, in part, to the elevated expression level of xylose isomerase, which was accomplished through the multiple-copy integration of XYLA in the chromosome of the evolved strain.
Among the possible oilseeds, Camelina sativa, a member of the Brassicaceae family, is an attractive crop platform for such metabolic engineering, based on its low input cost and ease of transformation (Nguyen et al., 2013; Ruiz-Lopez et al., 2014).
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System metabolic engineering-based approaches, improvements in media formulation, and modified fermentation methods could provide the desired ethanol yields from these microorganisms growing on lignocellulose hydrolysates.
Here we discuss synthetic biology and metabolic engineering strategies based on the native biology of aerobic methylotrophs for developing synthetic strains grown on methanol, with Escherichia coli as the prototype.
In the last decade, metabolic engineering approaches based on the mathematical optimization have been used extensively for the analysis and manipulation of metabolic networks.
To overcome these limitations, we have developed a new approach (OptORF) for identifying metabolic engineering strategies based on gene deletion and overexpression.
In this study, we present a new optimization approach, OptORF, to identify metabolic engineering strategies based on a minimal number of metabolic and transcription factor gene deletions and metabolic gene overexpression, which couple biomass and biochemical production.
More suggestions(15)
metabolic response based
metabolic flux based
metabolic shift based
metabolic capacity based
metabolic model based
metabolic branching based
metabolic activity based
metabolic abnormality based
metabolic information based
metabolic route based
metabolic enzymotherapy based
metabolic resistance based
metabolic pathway based
metabolic state based
metabolic syndrome based
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