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Consequently, the microbial networks and metabolic dynamics of bioprocesses catalyzed by these ecosystems are highly complex, and their visualization is regarded as essential to bioengineering technology and innovation.
In this work we attempt to elucidate how microbiological succession proceeds in a semi-industrial bioleaching column test system, with emphasis in determining the identity of the microorganisms participating and the metabolic dynamics of carbon fixation pathways.
However, the metabolic dynamics of B. fibrisolvens, including CLA and CLNA production, is not well understood.
Therefore, we next analyzed the metabolic dynamics of LNA, which is more unsaturated than LA, using MDT-10.
We have developed RT-MT to understand and evaluate the metabolic dynamics of several bacterial strains (Fig. 1).
As a single probiotic bacterium can exert several effects on human health mainly through the improvement of host innate immune responses by producing several bioactive substances[16] [21], an understanding of the metabolic dynamics of purified bacterium is absolutely essential.
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In contrast to classical biochemical approaches that often focus on single metabolites, metabolomics is emerging as a powerful tool in CNS research involving the collection of quantitative data on a broad series of metabolites in an attempt to gain an overall understanding of metabolism and/or metabolic dynamics associated with conditions of interest [ 142].
Metabolomics is a fast growing field, which conveys a snapshot of the metabolic dynamics or metabolic phenotype of the living organism whether healthy or in response to pathophysiological stimuli, environmental factors or disease.
RT-MT is expected to improve our understanding of metabolic dynamics that would be necessary for the extraction of particular characteristics of metabolic changes.
Thus, to improve bacterial production of bioactive substances, a solid understanding of metabolic dynamics is important.
Conventional approaches make intensive use of model-based studies, such as exploiting post-pulse metabolic dynamics after proper perturbation of the microbial system.
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