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Here we have proposed three simple steps to meaningful measures of bacterial metabolic activity based on the autofluorescence of NADH.
After each decontamination procedure the bacteria were incapable of proliferation, and showed no evidence of metabolic activity based on the generation of adenosine triphosphate (ATP).
The measurement of metabolic activity based on the extracellular acidification rate has attracted wide interests in the field of biochemical detection.
For instance, using [18F]-fluorodeoxyglucose (FDG) positron emission tomography (PET), researchers can assess brain metabolic activity based on the rate at which different regions take up the radioactive probe.
MTT assay was employed to estimate the total cellular metabolic activity based on the reduction of MTT by mitochondrial dehydrogenases.
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The biocompatibility was assessed by a metabolic activity assay based on MTT, where an increase in metabolic activity is interpreted as cell proliferation.
These adjusted values were then used to create two physical activity indices (one for weekday and one for weekend day activity) based on the metabolic equivalent task units (METS) set for each activity intensity category (one MET is defined as the energy expended while sitting quietly, equal to 3.5 ml O2/kg/min)[ 24].
A metabolic equivalent (MET) score was assigned to each sports activity based on the compendium of physical activities.
The potential increase in NOS activity based on the network analysis of the metabolic profile supported the use of bevacizumab (Avastin), an anti-VEGF drug.
Metabolic equivalent task scores 6, 4.5 and 2.5 were assigned respectively for vigorous, moderate and walking activity based on a compendium of physical activities (Ainsworth et al, 2000).
The residual metabolic activity was monitored based on the irreversible reduction of resazurin (7-hydroxy-3H-phenoxazin-3-one-10-oxide, blue) to resorufin (7-hydroxy-3H-phenoxazin-3-one, pink).
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metabolic network based
metabolic flux based
metabolic shift based
metabolic capacity based
metabolic model based
metabolic branching based
metabolic abnormality based
metabolic information based
metabolic route based
metabolic enzymotherapy based
metabolic resistance based
metabolic engineering based
metabolic state based
metabolic pathway based
metabolic syndrome based
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