Exact(2)
In Gene Expression Commons, raw microarray data is individually normalized against a large-scale common reference (for Drosophila genome, n = 2687 as of Nov 2013), mapped onto the probeset meta profile.
Secondly, structural homology to previously defined protein folds in Protein Data Bank (PDB) [ 51] or Pfam [ 19, 20] were searched for using a newly improved version of the meta-predictor, Meta-BASIC [ 52], which combines sequence profile, secondary structure, and prediction of the burial states of individual amino acid with various scoring systems and meta profile alignment algorithms.
Similar(58)
For human and mouse transcripts, all 3P-seq datasets for cell lines/tissues of each species were combined, after normalizing for the sequencing depth (i.e., number of uniquely mapping tags) of each dataset, to generate meta profiles.
The finding of a dip in enrichments over the TSS has been often reported before, e.g., it has been consistently found in meta profiles for most active histone marks as well as Pol II.
In case of the meta-profiles for the developmental stages, the Affymetrix meta-profile provides the average gene expression levels in all tissues during one developmental stage.
The meta-profile developmental stage analysis from the Affymetrix array platform indicates that these 19 genes were preferentially expressed during seedling stages (Figure2b and electronic Additional file2: Figure S1b).
Genevestigator developed a novel approach (meta-analysis) to assemble microarray data from different experiments into context-related profiles (meta-profiles).
This analysis will be expanded to include meta-profiles of genes expressed during the rice response to abiotic and biotic stresses and to hormone treatment.
We have also developed four meta-profiles for genes expressed in different tissues and at different developmental stages for each of the two platforms.
For 25 previously identified Hsfs, we integrated anatomical meta-profiling data from 983 Affymetrix arrays into a phylogenetic tree, revealing a global view of the functional redundancy within this family.
Through analyzing Affymetrix and Agilent 44K anatomic meta-profiles, 19 root-preferential genes were identified and their anatomic (Figure2a and2c; Electronic Additional file2: Figure S1a and c) and developmental expression patterns (Figure2b; Electronic Additional file2: Figure S1b, d and e) on both platforms are shown in Figure2 and electronic Additional file2: Figure S1.
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