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Moreover, no p43 messenger is detected in the control line because p43 is synthesized by the use of internal initiation site of translation occuring in the TRα1 transcript (Figure 1B).
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Galanin messenger was detected by in situ hybridization in all tumours investigated.
IRAC I, IRAC II, IxAC-B1, B3 and B5 messengers were detected in unfed females and the complete repertoire was detected in salivary glands from pooled females at days 3 and 5 of the bloodmeal.
The 3.5-kilobase messenger RNA was detected in mammalian brains and human thymus.
A 33-kilodalton membrane protein, termed VAP-33 (VAMP-associated protein of 33 kilodaltons), was identified whose corresponding messenger RNA was detected only in the central nervous system and the gill of Aplysia.
A 66 bp domain (62% G+C) of tmRNA (10Sa RNA; transfer messenger RNA) was detected that corresponded to the ssrA gene that encodes tmRNA in all sequenced bacterial genomes.
Abundant full-length Slc9a6 messenger RNA was detected in cerebral cortex from wild-type mice.
Messenger RNA was detected using a PCR-based method (Zhou et al., 2006) with minor modifications as follows: RNA extracted from samples was reverse transcribed using oligo-dT primers and analysed by touchdown PCR.
Nevertheless, new PKC- and cGMP-dependent ion channels were identified, and interactions between the second messenger systems were detected, hinting at novel mechanisms of sensitivity modulation in the insect olfactory system.
Messenger RNA was detected by RT-PCR using ReverTra Ace qPCR RT Master Mix (Toyobo, Osaka, Japan) with 50 ng total RNA, EagleTaq Master Mix (Roche Applied Science, Tokyo, Japan), and corresponding primer sets.
Since Flag TDP-43 messenger RNA was detected in the spinal cord contralateral to the injected side by real-time polymerase chain reaction analysis, the AAV virus was shown to spread contralaterally through the spinal cord causing motor paresis and reduction of compound muscle action potential size in the opposite forelimb.
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