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RNA messenger expression in the uterus from rats pregnant on days 4, 5 and 6 of gestation was analyzed by real time RT-PCR.
In line with this consideration, it is possible that this different timing in messenger expression impacts the protein levels at 2 dpf.
SWAP70 sh1 and sh3 sequences effectively impaired SWAP70 messenger expression, when compared with that of either the control (shC) or parental cell lines and were thus used in subsequent experiments.
Furthermore, in different solid tumours, variable MMP-2 protein amounts with insignificant expression of MMP-2 mRNA have been shown and correlated to feedback mechanisms, being able to shut off messenger expression after the secretion and/or binding of the protein [ 32].
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Messenger RNA expression analysis was performed using gene expression arrays on matched cervical mononuclear cells and peripheral blood mononuclear cells.
That study was performed using a less sensitive PCR approach, however, because PCR products were stained using ethidium bromide, and no attempt was made to quantify ER variant messenger RNA expression relative to WT-ER messenger RNA expression.
FGL2 messenger RNA expression correlated with EP3-6 and inducible nitric oxide synthase expression in all samples combined (r = 0.55, P <.0001; and r = 0.67, P <.001, respectively).
Klotho protein was expressed in vivo, and protein and messenger RNA expression decreased under the hypoxic condition.
Messenger RNA expression indexes were used as independent variable to describe the dependent variable – i.e. the miRNA expression.
FGL2 messenger RNA expression was determined with the use of semiquantitative polymerase chain reaction and was compared with previously determined messenger RNA expression levels for EP3-6 and inducible nitric oxide synthase for the same samples.
Bacterial endotoxins secreted from Gram-negative organisms and Staphylococcus aureus alpha toxin increase the BNP messenger RNA expression.
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