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Rather, it led them to express molecular markers that are characteristic of epithelial, mesodermal and pluripotent cells, all at the same time.
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hESC supplementation with BMP antagonist Noggin and bFGF produced a predominantly neuronal cell phenotype with extremely low expression of pluripotent, mesodermal and endodermal-specific genes.
Embryonic stem cells (ESCs) and somatic cell-derived induced pluripotent stem cells (iPSCs) possess unlimited self-renewal capacity and great potential to differentiate to various cell types of three embryonic germ layers (ectodermal, mesodermal, and endodermal).
They can proliferate extensively and have the ability to differentiate into endodermal, mesodermal, and ectodermal derivatives.
It acts in part by regulating the cohesion of mesodermal and endodermal (mesendodermal) progenitor cells [3].
To examine this possibility we analyzed tumor samples with an array of mesodermal and endodermal markers.
"Non-epithelial" kinase genes were associated with cytoskeleton organization, cell adhesion, mesodermal and epidermal development.
First, spherical cell EB aggregates were generated that contain ectodermal, mesodermal and endodermal derivatives (Primary Differentiation).
We double labeled enhancer-reporter constructs with mesodermal and founder- cell markers to verify this.
Mesodermal and muscle expression of either gars P234KY construct reduced survival, with mesodermal expression leading to earlier lethality than muscle at both temperatures.
Mice Zfp191 transcript is detected early during embryogenesis in ectodermal, endodermal, mesodermal and extra-embryonic tissues.
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