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The labeled cells were filtered through 30-μm pore size mesh to remove cell aggregates, and then sorted through a separation LS column attached to a MidiMACS separator (Miltenyi Biotec).
After washing with a cold medium, the cell suspension was passed through a 200 G mesh to remove large debris.Two-10 x 10 cells were then suspend in ice-cold Hank's balanced salt solution (Gibco) containing 0.5% bovine serum albumin (Gibco).
An adaptive mesh update procedure is also proposed for effective management of the mesh to remove, add and repair elements, since the computational mesh nodes move according to the flow.
This means in a few of the world's more epicurean watering holes you may witness young bartenders shaking drinks without ice or loading in large hand-hewn chunks, with less surface area to melt, then shaking furiously but briefly and "double straining" through fine mesh to remove any rogue ice particles.
Disrupted pancreas was filtrated through 100-μm mesh to remove other tissues.
Soil samples were sieved through 2 mm mesh to remove stones, roots, and large organic residues.
Similar(12)
We use a conservative finite volume approach of merging cells on a structured O-mesh to remove this time step limit imposed by the CFL condition.
Then, pollen samples were sieved through an appropriate set of meshes to remove floral debris.
Use a semi-fine mesh strainer to remove any orange pieces, while still allowing some tiny charred bits to make it into the final syrup.
The soil was mixed and passed through a 2 mm mesh sieve to remove stones, debris and plant material.
Add the sugar, a teaspoon at a time, making sure the sauce is not too sweet.
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