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Each sample was sieved using a 0.84 mm mesh filter for annual dose analysis (300 g).
To form double-layer microcapsules, alginate microcapsules were immersed in cationic dextran spermine solution (0.05 % w/v) in saline, that was filtered by 0.22 µm mesh filter, for 5 min (Ponce et al. 2006).
When aggregates formed with d = 7 μm backpacks are forced through a mesh filter for varying pore sizes, aggregates will dissociate and reassociate.
Splenocytes were obtained by mashing the whole spleen through a 70 μm mesh filter for immediate storage at −80°C in 90% (vol/vol) fetal calf serum and 10% (vol/vol) dimethylsulphoxide.
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In addition to the Slayer, hot coffee drinks can be brewed by a siphon method for a light body and flavor, through the fine mesh filter of a Trifecta brewer, for full-bodied flavor.
In addition, he said many consumers had started brewing coffee in reusable metal-mesh filters for the Keurig machines, which accept ground coffee.
As a second test, we compared the performance of our filter to that of two other filters: a combination of previously selected sex-specific MeSH (Table 1) (SS MeSH filter) and the previously published filter for retrieving information on women's health (M&S filter) (see Table 2[ 2[]).
Cell suspension was filtered through a mesh filter (40 µm pore size), spun for 5 min at 400 g, 4 °C, and resuspended in a small volume of RPMI-1640.
Dissociated cells were filtered through a 100 μm mesh filter and centrifuged at 100× g for 5 min in a sealed centrifuge (C3i centrifuge, Jouan Laboratories, Saint-Herblain, France).
Three grams of this subsample was mixed with 100 ml of sterile dispersant solution (0.15 M NaCl) and shaken for 120 s to disperse microbes into the liquid phase and filtered with a 30-μm pore-size nylon mesh filter (Type NY30, Millipore, Bedford, MA), for a total of 24 composite soil subsamples analyzed for Enterococcus.
This single cell suspension was passed through a 70 micron mesh filter and at this point was ready for FACS immunostaining as outlined above.
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