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We detected >17 million total variants upon merging the variant calls from the two libraries.
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To compare and merge the variants found by the two pipelines, we used vcflib vcfallelicprimitives (https://github.com/ekg/vcflib, last accessed December 11 , 2015 to decompose complex variants into single-nucleotide polymorphisms (SNPs) and indels.
After merging the unfiltered variant calls, the program creates a data set for each input method from annotations generated by the callers coupled with annotations computed by VariantMetaCaller.
The database was created by merging the FASTA format files of SwissProt, TrEMBL and their associated splice variants.
Merging the thickened crowns.
Two samples (01 and 03) did not meet our criteria for total number of bases (9 GB) with one Ion Proton I chip experiment; therefore, we repeated the Ion Proton run with samples from the same library and merged the two results before the variant calling (Table 2 and Methods).
The Hartz III reform in 2004 merged these variants into one subsidy (that is still in place today and is described above) for people with placement difficulties.
Copy-number variant regions were generated by extending variant windows and merging neighboring variant regions.
Finally, we argue that our approach and findings are consistent with other studies that note benefits of replicate exome comparisons for variant detection and replicated exome merging for variant calling accuracy [ 31].
Prior to merging, overlapping variants between the WES and chip data were removed from chip datasets.
Flatten your image (merge the layers).
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