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Second, cstacks was used to build a catalog of all existing loci and alleles after merging loci from multiple individuals.
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Loci were then merged across all individuals within each family with ' RADmarkers', merging loci with shared RAD alleles, allowing up to three mismatches between alleles.
The between-individual distance parameter of cstacks allows for mismatches while merging loci into the Catalog.
Most Mt3.5 genes (74%) are unchanged or found with only minor changes in structure in Mt4.0; 20% are involved in a merging of two loci (70% of merged loci originated from unanchored contigs, which were likely partial gene models in Mt3.5); splits and more complex associations account for the remaining 6% of Mt3.5 gene loci.
The cstacks program reads the output from ustacks and merges loci into the Catalog.
If each parent is fixed for a different allele at a particular locus, cstacks can detect the mismatch and properly merge the loci.
However, the distribution of error-based alleles is stochastic, whereas merged paralogous loci should produce population-wide shared polymorphism.
They also designed an algorithm for merging maps from different studies on the basis of loci common to different maps.
Subsequently, a 'synthetic' map is constructed by merging information on locus positions from component maps.
A cluster who broadcasts a merging request would not accept a merging request from other clusters.
These settings 1) optimize the recovery of a large number of loci while reducing the SNP and RAD allele error rates, and 2) filter a fraction of putative paralogous loci merged as a single locus (Mastretta-Yanes et al. 2014b).
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